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dc.contributor.authorPoyraz, İsmail
dc.date.accessioned2021-09-22T07:34:18Z
dc.date.available2021-09-22T07:34:18Z
dc.date.issued2014en_US
dc.identifier.citationİ. POYRAZ, “An Efficient DNA Isolation Method from Nigella sativa L Ranunculaceae Seeds for RAPD and ISSR Analysis,” Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, vol. 1, no. 1, pp. 22–27, Jun. 2014.en_US
dc.identifier.issn2148-2330
dc.identifier.urihttps://hdl.handle.net/11552/2014
dc.description.abstractNigella sativa L. (Ranunculaceae) seeds (black cumin) that contain high oil, terpene, protein, alkaloids are an economically important for medicine and food industry. Lack of an efficient DNA isolation procedure is a limiting factor for any molecular studies of these plant. We have used a genomic DNA isolation protocol for Nigella sativa seeds based on a hexadecyltrimethylammonium bromide (CTAB) method described for other plant species. The method is a modified CTAB extraction using high salt concentrations and polyvinyl pyrrolidone (PVP), and successive isoamyl alcohol chloroform extractions and involves mortar grinding of black seeds. Black seeds include little amount of DNA despite high amounts nutrients as polysaccharides and oils. Therefore, the yield was approx., 20 ng DNA per 150 mg of dry seed material. The genomic DNA obtained by this method was suitable to be used in inter-simple sequence repeat (ISSR) and random amplified-polymorphic DNA (RAPD) analyses. This extraction method should facilitate the molecular analysis as genotypic selection via use of dry seeds instead of germination of N. sativa seeds.en_US
dc.language.isoengen_US
dc.publisherBilecik Şeyh Edebali Üniversitesien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCTABen_US
dc.subjectDNA Extractionen_US
dc.subjectISSRen_US
dc.subjectNigella Sativa L.en_US
dc.subjectPAPDen_US
dc.subjectSeeden_US
dc.titleAn Efficient DNA Isolation Method from Nigella sativa L Ranunculaceae Seeds for RAPD and ISSR Analysisen_US
dc.typearticleen_US
dc.relation.ispartofBilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisien_US
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.authorid0000-0003-3651-5885en_US
dc.identifier.volume1en_US
dc.identifier.issue1en_US
dc.identifier.startpage22en_US
dc.identifier.endpage27en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthorPoyraz, İsmail


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