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dc.contributor.authorŞahin, Samet
dc.contributor.authorWongnate, Thanyaporn
dc.contributor.authorChuaboon, Litavadee
dc.contributor.authorChaiyen, Pimchai
dc.contributor.authorYu, Eileen Hao
dc.date.accessioned2022-10-11T14:06:15Z
dc.date.available2022-10-11T14:06:15Z
dc.date.issued2018en_US
dc.identifier.citationŞahin, S., Wongnate, T., Chuaboon, L., Chaiyen, P. and Yu, E.H., 2018. Enzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalyst. Biosensors and Bioelectronics, 107, pp.17-25.en_US
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.urihttps://doi.org/10.1016/j.bios.2018.01.065
dc.identifier.urihttps://hdl.handle.net/11552/2633
dc.description.abstractIn enzymatic fuel cells (EnFCs), hydrogen peroxide formation is one of the main problems when enzymes, such as, glucose oxidase (GOx) is used due to the conversion of oxygen to hydrogen peroxide in the catalytic reaction. To address this problem, we here report the first demonstration of an EnFC using a variant of pyranose-2-oxidase (P2O-T169G) which has been shown to have low activity towards oxygen. A simple and biocompatible immobilisation approach incorporating multi-walled-carbon nanotubes within ferrocene (Fc)-Nafion film was implemented to construct EnFCs. Successful immobilisation of the enzymes was demonstrated showing 3.2 and 1.7-fold higher current than when P2O-T169G and GOx were used in solution, respectively. P2O-T169G showed 25% higher power output (maximum power density value of 8.45 +/- 1.6 mu W cm(-2)) and better stability than GOx in aerated glucose solutions. P2O-T169G maintained >70% of its initial current whereas GOx lost activity >90% during the first hour of 12 h operation at 0.15 V (vs Ag/Ag+). A different fuel cell configuration using gas diffusion cathode and carbon paper electrodes were used to improve the power output of the fuel cell to 29.8 +/- 6.1 mu W cm(-2). This study suggests that P2O-T169G with low oxygen activity could be a promising anode biocatalyst for EnFC applications.en_US
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey - (TUBITAK). Türkiye Bilimsel ve Teknolojik Araştırma Kurumu - (TÜBİTAK). UK Research & Innovation (UKRI). Engineering & Physical Sciences Research Council (EPSRC) - EP/H0194801. Thailand Research Fund (TRF) - RTA5980001 / MRG6080234. Royal Golden Jubilee Scholarship - PHD/0172/2556.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.identifier.doi10.1016/j.bios.2018.01.065en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiosensoren_US
dc.subjectEnzymatic Fuel Cellsen_US
dc.subjectPyranose-2-oxidaseen_US
dc.subjectGlucose Oxidaseen_US
dc.subjectFerroceneen_US
dc.subjectNafionen_US
dc.titleEnzymatic fuel cells with an oxygen resistant variant of pyranose-2-oxidase as anode biocatalysten_US
dc.typearticleen_US
dc.relation.ispartofBiosensors and Bioelectronicsen_US
dc.departmentFakülteler, Mühendislik Fakültesi, Kimya Mühendisliği Bölümüen_US
dc.authorid0000-0002-0568-4283en_US
dc.identifier.volume107en_US
dc.identifier.startpage17en_US
dc.identifier.endpage25en_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.indexPubMeden_US
dc.relation.indexScopusen_US
dc.relation.indexWoSen_US
dc.relation.indexWoS - Science Citation Index Expandeden_US
dc.contributor.institutionauthorŞahin, Samet
dc.description.wospublicationidWOS:000428006900003en_US
dc.description.scopuspublicationid1-s2.0-S0956566318300794en_US
dc.description.pubmedpublicationidPMID: 29427882en_US
dc.authorwosidAAD-7966-2020en_US
dc.description.wosqualityQ1en_US


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