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dc.contributor.authorDarcan, Cihan
dc.date.accessioned2023-04-13T11:57:21Z
dc.date.available2023-04-13T11:57:21Z
dc.date.issued2012en_US
dc.identifier.citationDarcan, C. (2012). Expression of OmpC and OmpF porin proteins and survival of Escherichia coli under photooxidative stress in Black Sea water. Aquatic Biology, 17(2), 97-105.en_US
dc.identifier.urihttps://doi.org/10.3354/ab00458
dc.identifier.urihttps://hdl.handle.net/11552/2946
dc.description.abstractIn the present study, the synthesis of OmpC and OmpF porins (outer membrane proteins C and F, respectively) of Escherichia coli under photooxidative stress in Black Sea water and the roles of RpoS (the starvation/stationary phase sigma factor, σ38) and EnvZ (a transmembrane environmental sensor protein) in changing porin protein expression were investigated. The t99 (time required for a 2 log reduction) values for wild-type E. coli W3110 and MSZ31 (envZ−) under photooxidative stress were observed to be 14.3 and 16.8 h, respectively, while for porin-deficient mutants MKC505 (ompC−), MH621 (ompF−), MKCF36 (ompF−ompC−), and MSR31 (ompR−) they were 10.6, 10.2, 9.8 and 9.1 h, respectively. Further, when the porins or ompR were deleted, the resistance to photooxidative stress decreased. OmpC and OmpF porin expression increased in all studied transcriptional fusion strains (wild type and mutants) in control microcosms, and almost completely decreased under photooxidative stress in the test microcosms, which consisted of light exposure with methylene blue added as a photosensitizer. Thus, it could be concluded that RpoS and EnvZ regulation of the synthesis of OmpC and OmpF is not photooxidation-dependent. This study determined for the first time that the expression of ompC in brackish water is influenced by RpoS.en_US
dc.language.isoengen_US
dc.publisherInter-Research Science Publisheren_US
dc.identifier.doi10.3354/ab00458en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPorinen_US
dc.subjectPhotooxidationen_US
dc.subjectEscherichia colien_US
dc.subjectBlack Seaen_US
dc.subjectRpoSen_US
dc.subjectEnvZen_US
dc.titleExpression of OmpC and OmpF porin proteins and survival of Escherichia coli under photooxidative stress in Black Sea wateren_US
dc.typearticleen_US
dc.relation.ispartofAquatic Biologyen_US
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.authorid0000-0003-0205-3774en_US
dc.identifier.volume17en_US
dc.identifier.issue2en_US
dc.identifier.startpage97en_US
dc.identifier.endpage105en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.indexScopusen_US
dc.relation.indexWoSen_US
dc.relation.indexWoS - Science Citation Index Expandeden_US
dc.contributor.institutionauthorDarcan, Cihan
dc.description.other1Q2en_US
dc.description.wospublicationidWOS:000312246300001en_US
dc.description.wosqualityQ2en_US


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