The bioactive properties of a bryophyte collected from Bilecik (Turkey) Province

Abstract

Studies on the discovery of natural bioactive substances and the determination of their potential use in drug synthesis have gained importance due to the toxicity of existing chemical drugs and the increasing antibiotic resistance of pathogenic bacteria. In this context, bioactive secondary compounds synthesized by bryophyte samples collected from different geographic locations attract great interest. In this study, the antimicrobial, antioxidant, and anticancer activities of the extracts obtained from the bryophyte sample were investigated after the identification of the bryophyte sample collected from Bilecik Province. Agar Disk Diffusion (ADD) and Minimal inhibitory concentration (MIC) methods were used to determine the antimicrobial activity. DPPH removal activity and (3,4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide (MTT) were carried out for the determination of the antioxidant capacity and anticancer activity, respectively. According to the results of the current study, the extracts of the bryophyte species, defined as Leucodon sciuroides (Hedw.) Schwaeurogr, prepared with ethyl acetate and hexane, was found to have no significant antimicrobial and antioxidant activity, but methanol extract was found to have antifungal potential against C. albicans species. The ethyl acetate and methanol extracts from the bryophyte sample showed a higher antiproliferative effect against MCF-7 (ATCC number HTB-22TM) (IC50: 51.16 and 98.17) and MDA-MB-231 (ATCC number HTB26TM) (IC50: 20.21 and 15.47). However, the same extract could not 50% inhibit the healthy cell line (3T3) up to the maximum concentration, >500 mg/mL. As a result, L. sciuroides has the potential to be used in the synthesis of new compounds with natural and selective toxicity.(c) 2023 SAAB. Published by Elsevier B.V. All rights reserved.