MOLECULAR CHARACTERIZATION OF SERVICE TREE (Sorbus domestica L.) GENOTYPES SELECTED FROM TOKAT PROVINCE

Abstract

This study was carried out to determine the genetic diversity of eleven service tree (Sorbus domestica L.) genotypes selected from Tokat province and taken under protection in the genetic resource plot. In the present study, ISSR-PCR studies were carried out on genotype DNAs obtained by Mini-CTAB method and the level of polymorphism between genotypes was determined using seven UBC-ISSR primers. A total of 65 bands were obtained in service tree genotypes with seven primers and 56 of these bands were determined to be polymorphic. The number of bands obtained per primer varied between 5 and 12, the mean band number was 5.90, and the mean polymorphic band number was 5.09. The data obtained as a result of the evaluation of ISSR-PCR bands were analyzed in Popgene32 and MEGA5.0 computer package program, and a dendrogram was obtained according to the UPGMA method. In the dendrogram obtained, it was determined that two groups were formed at the level of approximately 22% difference, and there was only one genotype in the small group while the other genotypes were in the large group.