Kribbella soli sp nov., isolated from soil

Abstract

A novel actinobacterial strain, designated FMN22(T), was isolated from soil and characterised using a polyphasic approach. Strain FMN22(T) showed high 16S rRNA gene sequence similarity to Kribbella karoonensis Q41(T) (99.3%), Kribbella shirazensis UTMC 693(T) (99.0%), Kribbella aluminosa HKI 0478(T) (98.9%) and Kribbella hippodromi S1.4(T) (98.6%). Phylogenetic analysis using the 16S rRNA and concatenated gene (gyrB, rpoB, relA, recA and atpD) sequences showed that strain FMN22(T) is closely related to the type strains of K. karoonensis DSM 17344(T), K. shirazensis UTMC 693(T), K. aluminosa HKI 0478(T), K. hippodromi S1.4(T), Kribbella jejuensis HD9(T) and Kribbella solani DSA1(T). Based on concatenated gene genetic distances analysis, strain FMN22(T) is distinct from all Kribbella type strains. DNA-DNA hybridization experiments with closely related type strains, were found to be 59.2 +/- 2.4, 54.8 +/- 2.1, 16.4 +/- 2.3 and 38.6 +/- 2.5%, relatedness to K. karoonensis DSM 17344(T), K. shirazensis DSM 45490(T), K. aluminosa DSM 18824(T) and K. jejuensis DSM 17305(T), respectively. The cell wall peptidoglycan contained ll-diaminopimelic acid, and whole cell sugars were glucose, mannose and ribose. The predominant menaquinone was MK-9(H-4). The major phospholipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. Major fatty acids are anteiso-C-15:0 and iso-C-16:0. These chemotaxonomic traits are in good agreement with those known for representatives of the genus Kribbella. A combination of DNA-DNA hybridization results and phenotypic properties demonstrated that strain FMN22(T) can be clearly distinguished from all close phylogenetic relatives. Therefore, strain FMN22(T) (=DSM 27132(T) = KCTC 29219(T)) is considered to be the type strain of a novel species of the genus Kribbella, for which the name Kribbella soli is proposed.