Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model

Abstract

Objectives: This study was conducted to research the protective effects of Morinda citrifolia (Noni) against the neuro- toxicity, of 6-Hydroxydopamine (6-OHDA)-induced in the neuroblastoma (SH-SY5Y) cells model. Methods and Results: 200 μM 6-OHDA were applied on SH-SY5Y cells under in vitro conditions to model Parkinson’s Disease. Before the 6-OHDA application, it was treated with different doses of Noni extract (5, 10, 25, and 50 μg/mL). Cell viability was measured with routinely used 3-(4,5-Dimethyl Thiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and cytotoxic activity was determined by lactate dehydrogenase (LDH) assays. Besides cell viability, oxidative stress was evaluated by total antioxidant capacity (TAC), total oxidant status, superoxide dismutase assay, malondialdehyde, and glutathione reductase. The effect of Noni on Bax/Bcl2 gene regulation in SH-SY5Y cells was investigated by real-time PCR. 6-OHDA 200 μM decreased cell viability by approximately 60% in SH-SY5Y cells and increased LDH level. With 50 μg/mL Noni treatment, cell viability increased to 88.32%, while the LDH level decreased. Similarly, Noni re-regulated Bax and Bcl-2 level, modified 6-OHDA, and decreased oxidative stress levels (p<0.05 and p<0.001). Conclusion: These findings prove that noni exhibits a neuroprotective effect against 6-OHDA induction, through Bax/ Bc-l2 gene regulation and decreasing oxidative stress.