Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Abstract

Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration (MIC) values were determined on Streptococcus mitis at doses of 31.25 mu g/ml of Sodium Perborate Metahydrate (SPM), Zinc Borate (ZB) 62.5 mu g/ml and Etidote 125 mu g/ml. Etidote+SPM, Etidote+ZB, and ZB+SPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 mu g/ml+32 mu g/ml in all combinations, while it showed an effect on biofilm at doses of 1028 mu g/ml+ 1028 mu g/ml HepG2 Cell culture.