The genetic relationship between some strawberry cultivars and F1 population derived from these cultivars

Abstract

this study, Osmanli strawberry cultivar that has specific and superior characteristics was used as a maternal parent to create crosses with three local ('Karacilek', 'Tuylu', and 'Deli') and three standards ('Kabarla', 'Sweet Ann' and 'Sweet Charlie') strawberry cultivars as paternal parents. Fifty-two F-1 genotypes resulting from these hybridization studies, their parents were selected for studying the genetic distances among them and with Fragaria chiloensis. Fourteen UBC-ISSR primers in which polymorphism levels were determined, were used. 76 DNA bands were obtained in total and 60 of them were polymorphic. After the evaluation of ISSR-PCR bands, the data were analyzed in Popgene32 and MEGA5.0 packaged software and a dendrogram was obtained according to the UPGMA method. The dendrogram was separated into two major groups at the 20% difference level, one large and one small group. Fragaria chiloensis, 'Tuylu-2' local cultivar, CC48, and CA97 F1 genotypes participated in the small group while the other F1 genotypes and parents participated in the large group. This suggests that 'Tuylu' local cultivars were closer to F. chiloensis than most genotypes issued from 'Osmanli' cultivar.