The Antimicrobial Activities of the Leaves of Some Endemic Stachys Species spreading in West Anatolia, Turkey

Abstract

The extracts of the leaves of six endemic Stachys sp. (Lamiaceae) which were collected from Turkey were investigated for their antimicrobial activities. In this study, the antimicrobial activity of ethanol, aceton and hexane extracts of Stachys annua subsp. cilicia, S. setifera subsp. lycia, S. sosnowskyi, S. tmolea, S. cretica subsp. anatolica, ve S. iberica subsp. iberica var. densipilosa species which were collected from Kütahya and Eskişehir cities were investigated on some microorganisms. The effect of extracts on S. aureus ATCC 6535, S. epidermidis ATCC 11228, B. cereus, Gram (-) E. coli W3110, P aeruginosa ATCC 27853, P. vulgaris ATCC 13315 were investigated by using disc diffusion and microbroth dilution methods. Antibiotics were used as positive controls. According to the results, while hexane and aceton extracts of endemic Stachys species exhibited no antibacterial activities, ethanol extracts of 3 endemic species (S. tmolea, S. cretica subsp. anatolica, S. iberica subsp. iberica var. densipilosa) showed a strong antibacterial activity, especially Gram (+) Sthaphylococcus aureus. According to the results, it was observed that the Stachys extracts are more effective against Gram (+) bacteria. The results demonstrated that the ethanol extract of the leaves of S. tmolea, S. cretica subsp. anatolica, S. iberica subsp. iberica var. densipilosa has a strong antimicrobial activity. The secondary metabolites isolated from endemic Stachys sp. could be used in an effective manner to fight especially against S. aureus.