The Extract of Momordica charantia Inhibits Cell Proliferation and Migration in U87G Cells

Abstract

Glioblastoma multiforme is the most common brain cancer among central nervous system tumors. Although there are various treatment methods, these methods provide limited. Therefore, there is an urgent need for new more effective treatment strategies. This study, it was aimed to investigate the effect of Momordica charantia fruit extract on cell proliferation and migration using glioblastoma cells. The cells were treated with different concentrations of fruit extract and viability was measured by MTT analysis. The anti-migration effect of fruit extract on the cells was demonstrated by Scratch analysis. Also, the protein expression of Sparc and Src was investigated via the Western blot technique. Treatment of the fruit extract significantly resulted in decreased cell proliferation and the half-maximal inhibitory effect was detected as 700 mu g/mL for 24 h. It inhibited cell migration ability and increased wound width on time-depending. Moreover, reductions in Sparc and Src protein expression levels were observed. The fruit extract exhibited a cytotoxic role by reducing proliferation and wound healing ability in glioblastoma cells depending on the concentration. Considering all the results, we proposed that inhibition of cell proliferation and migration is due to its effect on the expression of Src and Sparc proteins.