Green synthesis of silver nanoparticles and designing a new amperometric biosensor to determine glucose levels

Abstract

The high-level of glucose in daily nutrition causes diabetes (Diabetes mellitus) and obesity. It is also important to determine glucose levels in food production processes for quality control methods. Biosensors are bio analytical devices that provides cheap, simple, analyses and have a fast response time. Silver nanoparticles are used to modify electrodes in bio-sensing glucose. The nanoparticles could be synthesized via the green synthesis technique. In this technique, plants could be used both reducing and stabilizing agents. Agricultural waste, an environmental problem, is increasing with the population. These wastes are a good natural source and have the potential for green synthesis of silver nanoparticles to use in biosensor applications. In this study, a new amperometric glucose biosensor was designed. For this purpose, carbon paste electrode (CPE) was modified by green synthesized waste tea based silver nanoparticles (WT-AgNPs) and the glucose oxidase enzyme was immobilized on MCPE by the cross -linking. Glucose determination was performed based on the reduction of hydrogen peroxide at +0.4 V versus Ag/AgCl. The linear working range was determined as 0.10-1.0 mu M. In addition, the designed biosensor was applied to detect the amount of glucose in a commercial fruit juice. The results showed that the designed biosensor has low detection limit, very good reproducibility, selectivity, and almost long shelf life.