Conducting polymer-based electrochemical biosensor for the detection of acetylthiocholine and pesticide via acetylcholinesterase

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dc.authoridGoktas, Hasan/0000-0002-2195-9531
dc.authoridISIK, MESUT/0000-0002-4677-8104
dc.authoridAKDAG, ABDURRAHMAN/0000-0001-5292-8001
dc.contributor.authorAkdag, Abdurrahman
dc.contributor.authorIsik, Mesut
dc.contributor.authorGoktas, Hasan
dc.date.accessioned2025-05-20T19:00:51Z
dc.date.issued2021
dc.departmentBilecik Şeyh Edebali Üniversitesi
dc.description.abstractA voltammetric biosensor for acetylthiocholine (ATCh) and paraoxon detection was successfully developed. To achieve this goal, polypyrrole (PPy) was synthesized onto the platinum (Pt) electrode surface in 0.30 M oxalic acid solution containing 25 mM pyrrole. PPy-coated Pt (Pt/PPy) electrode surface was covered with chitosan (Chi) (Pt/PPy/Chi). The acetylcholinesterase (AChE) enzyme was immobilized on the Pt/PPy/Chi electrode surface to build a voltammetric biosensor (Pt/PPy/Chi/AChE). The storage stability of the biosensor was determined to be 72% even after 60 days. The operational stability was determined to be 94% after 20 consecutive measurements. For the biosensor, the linear range was determined to be 30-50 mu M for ATCh and 0.46-1.84 nM for paraoxon. The limit of detection (LOD) was determined to be 0.45 mu M for ATCh and 0.17 nM for paraoxon
dc.identifier.doi10.1002/bab.2030
dc.identifier.endpage1119
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.issue6
dc.identifier.pmid32941665
dc.identifier.scopus2-s2.0-85092530543
dc.identifier.scopusqualityQ2
dc.identifier.startpage1113
dc.identifier.urihttps://doi.org/10.1002/bab.2030
dc.identifier.urihttps://hdl.handle.net/11552/8798
dc.identifier.volume68
dc.identifier.wosWOS:000578756000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWoS
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakWoS - Science Citation Index Expanded
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250518
dc.subjectbiosensors
dc.subjectenzyme
dc.subjectimmobilization
dc.subjectpesticide
dc.subjectpolypyrrole
dc.titleConducting polymer-based electrochemical biosensor for the detection of acetylthiocholine and pesticide via acetylcholinesterase
dc.typeArticle

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