Investigation of Effects Combined Treatment with Caffeic Acid Phenethyl Ester and Propranolol on MDA-MB-231 Breast Cancer Cell Line

Abstract

Caffeic acid phenethyl ester (CAPE) is one of the active components of propolis with anti-cancer effects. Propranolol has been shown to be a protective effect in the development of cancer due to its antiangiogenesis and B-adrenergic receptor supression. Recently, drug combination studies have been widely used in the treatment of many fetal diseases such as cancer. Combined treatment can prefer both benefit and reduce side efects of patient. The aim of this study is to find the migration analyis of MDA-MB-231 breast cancer cell line with combined treatment of CAPE and Propranolol. In this study cell viabilty was measured by MTT assay. For wound healing, the MDA-MB-231 cell line was cultured to be 105 cells in 100 uL in a 6-well culture plate. After 90% of confluency, we applied 30 uM CAFE, 150 uM Propranolol and combined treatment at 15 uM CAPE and 75 uM Propranolol. We observed width of scar 0,24,48 and,72 hours with inverted microscope. When the data about wound closure rates were evaluated, it was observed that combined therapy inhibited migration of cancer cells compared to untreated cells and single therapy.